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EXPLORING THE TELOSOME’S POTENTIAL IN DIAGNOSIS AND PROGNOSIS OF PROSTATE CANCER.
Introdução, Material, Método, Resultados, Discussão e Conclusões
Introduction: The telosome is a protein complex composed of six subunits and responsible for telomere maintenance and regulating telomerase activity. In prostate cancer (PC), telomere dysfunction and telomerase reactivation are essential elements for the initiation and progression of the disease. Despite the direct involvement of the telosome in this process, little is known about the expression profile of its subunits in the neoplasia.
Objectives: In this work we studied the gene expression of five subunits of the telosome (POT1, TRF2, TPP1, RAP1 and TIN2) in PC. We also correlate these expressions with prognostic factors in PC.
Methods: Expression levels were evaluated in 60 surgical specimens of localized PC by Q-PCR. Ten samples of benign prostatic hyperplasia (BPH) were used as control. T test was used for statistical analysis. The low risk and high risk group are based in “D’Amico Risk Classification”, with 20 samples of PC in each group.
Results: We showed that the five telosome subunits are upregulated in PC when compared with control group (p≤ 0.05). POT1 are downregulated in patients with tumor volume smaller than 10cm³ (p= 0.0383) and in patients of low risk group when compare with high risk group (p = 0.0225). TRF2 are upregulated in patients with PSA <10 (p= 0.0157) and in patients of low risk when compare with high risk group (p= 0.0099). RAP1 are downregulated in patients with PSA <10 (p= 0.012) and in patients with clinical stage of T1-T2a (p=0.0137).
Conclusion: Our results showed that the telosome has an aberrant expression profile in PC, and is probably involved with the carcinogenesis process since we can differentiate between malignant and benign tissue. In addition, POT1, TRF2 and RAP1 have some potential as biomarkers in PC prognostic.
Telomere, Telosome, Biomarker
Câncer de Próstata Localizado
FMUSP - Sao Paulo - Brasil
Gabriel Arantes Galvão Dias dos Santos, Nayara Izabel Viana, Ruan Pimenta, Vanessa Ribeiro Guimarães, Juliana Alves de Camargo, Iran Amorim Silva, Katia Ramos Moreira Leite, Sabrina Reis, Miguel Srougi