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Título

SNP RS1834306 ON MICRORNA-100 IS ASSOCIATED WITH BETTER PROGNOSTIC IN PROSTATE CANCER

Introdução, Material, Método, Resultados, Discussão e Conclusões

Background: Prostate cancer (PCa) is the most common non-cutaneous tumor in the male population. In Brazil, according to the National Cancer Institute (INCA), approximately 65,840 new cases of this tumor are estimated in 2020, being the second most common type among men. MicroRNAs (miRs) interfere with the main cellular functions with properties closely linked to oncogenesis, and can act as protective miRs or as oncomiRs in cancer, including prostate cancer. Single Nucleotide Polymorphisms (SNPs) are characterized by a single nitrogenous base exchange in DNA, occurring in coding and nocoding regions. Considering that genetic factors are related to the appearance and evolution of PCa, it is believed that the identification of polymorphisms in miRs involved in PCa and the impact of these variations on the different stages of carcinogenesis of this tumor, could allow the development of markers for diagnosis and prognosis tumor. Objectives: To evaluate the role of the rs1834306 polymorphism located in miR-100 in the development and prognostic of PCa. In addition, correlate genotyping with classical PCa prognostic factors and with clinical data. Methods: The study consisted of whole blood samples from 100 patients diagnosed with PCa and the control group by 68 healthy individuals. The genotyping of the polymorphism selected for analysis was evaluated using the quantitative polymerase chain reaction technique in real time (qRT-PCR) and correlated with preoperative PSA, Gleason histological grading, pathological staging and biochemical recurrence. Results: The rs1834306 polymorphism (miR100) was identified in 70.6% and 75% in patients in the PCa and control groups, respectively. Regarding prognostic factors, we found that this SNP was significantly less present in the group of patients with PSA> 10ng / ml, both when considering the genotype and when considering only the polymorphic allele (p = 0.03 and p = 0.09, respectively). Conclusions: With our data we conclude that the presence of SNP rs1834306 in miR100 acts as a polymorphism related to the most favorable prognostic in prostate cancer.

Palavras Chave

Prostate cancer; Single Nucleotide Polymorphisms; microRNA; miR-100.

Área

Câncer de Próstata Metastático

Instituições

FMUSP - Sao Paulo - Brasil

Autores

Patricia Rodrigues Candido, Renan Eboli Lopes, Nayara Izabel Viana, Vanessa Schreiter Guimarães, Juliana Alves Camargo, Ruan Pimenta, Katia Ramos Moreira Leite, Miguel Srougi, Alberto Azoubel Antunes, Sabrina Thalita Reis